Genetic complementation between replication-defective mutants of HIV-1 and SIVagm
Identifieur interne : 001315 ( Main/Exploration ); précédent : 001314; suivant : 001316Genetic complementation between replication-defective mutants of HIV-1 and SIVagm
Auteurs : T. Miura [Japon] ; R. Shibata [Japon] ; A. Adachi [Japon] ; T. Kuwata [Japon] ; J. Chen [Japon] ; M. Jin [Japon] ; E. Ido [Japon] ; M. Hayami [Japon]Source :
- Archives of Virology [ 0304-8608 ] ; 1996-01-01.
English descriptors
- KwdEn :
- Adachi, Assay, Chimeric, Chimeric viruses, Clone, Compatibility, Complementation, Culture supernatants, Cultures cotransfected, Cytopathic effect, Functional comparison, Hayami, Human immunodeficiency virus, Human immunodeficiency virus type, Immunodeficiency, Immunodeficiency virus, Insertion, Kiyomasu, Linker insertion, Macaque, Macaque monkeys, Miura, Mutant, Primate lentiviruses, Proc natl acad, Replication, Retrovirus, Sakai, Shibata, Simian, Simian immunodeficiency virus, Sivagm, Sivagm mutants, Supernatant, Transfected, Transfection, Virol.
- Teeft :
- Adachi, Assay, Chimeric, Chimeric viruses, Clone, Compatibility, Complementation, Culture supernatants, Cultures cotransfected, Cytopathic effect, Functional comparison, Hayami, Human immunodeficiency virus, Human immunodeficiency virus type, Immunodeficiency, Immunodeficiency virus, Insertion, Kiyomasu, Linker insertion, Macaque, Macaque monkeys, Miura, Mutant, Primate lentiviruses, Proc natl acad, Replication, Retrovirus, Sakai, Shibata, Simian, Simian immunodeficiency virus, Sivagm, Sivagm mutants, Supernatant, Transfected, Transfection, Virol.
Abstract
Summary: To investigate the functional complementation of essential genes for virus growth between HIV-1 and SIVagm derived from African green monkeys, we co-transfected replication-defective molecular clones containing mutations ingag, pol, env, tat orrev, and monitored transient complementation by reverse transcriptase assay (RT), cytopathic effect (CPE) and immunofluorescence assay (IFA). The following results were obtained: 1) No complementation was observed in combinations of thegag andpol mutants. 2) Therev mutant of HIV-1 was minimally complemented by other SIVagm mutants, although therev mutant of SIVagm was significantly complemented by other HIV-1 mutants. 3) Among all combinations tested, theenv mutant of HIV-1 was the most effectively complemented by SIVagm mutants. 4) CPE was mostly absent in combinations of theenv mutant of SIVagm and thegag, pol ortat mutants of HIV-1, although there were significant positive results in RT and IFA assays. These findings provided basic information about the functional compatibility of pathogenic HIV-1 and nonpathogenic SIVagm which will be useful for generating chimeras of these two viruses.
Url:
DOI: 10.1007/BF01718586
Affiliations:
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Kuwata</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Immunodeficiency Viruses, Institute for Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Chen, J" sort="Chen, J" uniqKey="Chen J" first="J." last="Chen">J. Chen</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Immunodeficiency Viruses, Institute for Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Jin, M" sort="Jin, M" uniqKey="Jin M" first="M." last="Jin">M. Jin</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Immunodeficiency Viruses, Institute for Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Ido, E" sort="Ido, E" uniqKey="Ido E" first="E." last="Ido">E. Ido</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Immunodeficiency Viruses, Institute for Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Immunodeficiency Viruses, Institute for Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1996-01-01">1996-01-01</date><biblScope unit="volume">141</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="31">31</biblScope><biblScope unit="page" to="41">41</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adachi</term><term>Assay</term><term>Chimeric</term><term>Chimeric viruses</term><term>Clone</term><term>Compatibility</term><term>Complementation</term><term>Culture supernatants</term><term>Cultures cotransfected</term><term>Cytopathic effect</term><term>Functional comparison</term><term>Hayami</term><term>Human immunodeficiency virus</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Immunodeficiency virus</term><term>Insertion</term><term>Kiyomasu</term><term>Linker insertion</term><term>Macaque</term><term>Macaque monkeys</term><term>Miura</term><term>Mutant</term><term>Primate lentiviruses</term><term>Proc natl acad</term><term>Replication</term><term>Retrovirus</term><term>Sakai</term><term>Shibata</term><term>Simian</term><term>Simian immunodeficiency virus</term><term>Sivagm</term><term>Sivagm mutants</term><term>Supernatant</term><term>Transfected</term><term>Transfection</term><term>Virol</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adachi</term><term>Assay</term><term>Chimeric</term><term>Chimeric viruses</term><term>Clone</term><term>Compatibility</term><term>Complementation</term><term>Culture supernatants</term><term>Cultures cotransfected</term><term>Cytopathic effect</term><term>Functional comparison</term><term>Hayami</term><term>Human immunodeficiency virus</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Immunodeficiency virus</term><term>Insertion</term><term>Kiyomasu</term><term>Linker insertion</term><term>Macaque</term><term>Macaque monkeys</term><term>Miura</term><term>Mutant</term><term>Primate lentiviruses</term><term>Proc natl acad</term><term>Replication</term><term>Retrovirus</term><term>Sakai</term><term>Shibata</term><term>Simian</term><term>Simian immunodeficiency virus</term><term>Sivagm</term><term>Sivagm mutants</term><term>Supernatant</term><term>Transfected</term><term>Transfection</term><term>Virol</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: To investigate the functional complementation of essential genes for virus growth between HIV-1 and SIVagm derived from African green monkeys, we co-transfected replication-defective molecular clones containing mutations ingag, pol, env, tat orrev, and monitored transient complementation by reverse transcriptase assay (RT), cytopathic effect (CPE) and immunofluorescence assay (IFA). The following results were obtained: 1) No complementation was observed in combinations of thegag andpol mutants. 2) Therev mutant of HIV-1 was minimally complemented by other SIVagm mutants, although therev mutant of SIVagm was significantly complemented by other HIV-1 mutants. 3) Among all combinations tested, theenv mutant of HIV-1 was the most effectively complemented by SIVagm mutants. 4) CPE was mostly absent in combinations of theenv mutant of SIVagm and thegag, pol ortat mutants of HIV-1, although there were significant positive results in RT and IFA assays. These findings provided basic information about the functional compatibility of pathogenic HIV-1 and nonpathogenic SIVagm which will be useful for generating chimeras of these two viruses.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région du Kansai"><name sortKey="Miura, T" sort="Miura, T" uniqKey="Miura T" first="T." last="Miura">T. Miura</name></region><name sortKey="Adachi, A" sort="Adachi, A" uniqKey="Adachi A" first="A." last="Adachi">A. Adachi</name><name sortKey="Chen, J" sort="Chen, J" uniqKey="Chen J" first="J." last="Chen">J. Chen</name><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name><name sortKey="Ido, E" sort="Ido, E" uniqKey="Ido E" first="E." last="Ido">E. Ido</name><name sortKey="Jin, M" sort="Jin, M" uniqKey="Jin M" first="M." last="Jin">M. Jin</name><name sortKey="Kuwata, T" sort="Kuwata, T" uniqKey="Kuwata T" first="T." last="Kuwata">T. Kuwata</name><name sortKey="Shibata, R" sort="Shibata, R" uniqKey="Shibata R" first="R." last="Shibata">R. Shibata</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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